primary antibodies against card8 pab0281  (Abnova)

Journal: Scientific Reports

Article Title: Expression of CARD8 in human atherosclerosis and its regulation of inflammatory proteins in human endothelial cells

doi: 10.1038/s41598-020-73600-4

Figure Lengend Snippet: Immunostaining of CARD8 in non-atherosclerotic arteries. CARD8 expression was found in the endothelial layer of intima and smooth muscle cells in the media of non-atherosclerotic artery (Left: Artery from colon tissue, Magnification, × 30; Right: Popliteal artery, Magnification, × 10). Scale bar: 100 μm. Full images are available as Supplementary material.

Article Snippet: The staining was performed using primary antibodies against CARD8 (PAB0281; Abnova Corp, Taipei City, Taiwan; 1:1000/Nordic BioSite AB, Täby, Sweden), CD68 (NCL‐L‐CD68, Novacastra, Newcastle, UK; 1:50), VWF (M0616; Dako, Glostrup, Denmark; 1:50) and smooth muscle actin (SMA; M0851; Dako; 1:500) diluted in Da Vinci Green (Biocare Medical) and incubated for 1 h at room temperature.

Techniques: Immunostaining, Expressing

Journal: Scientific Reports

Article Title: Expression of CARD8 in human atherosclerosis and its regulation of inflammatory proteins in human endothelial cells

doi: 10.1038/s41598-020-73600-4

Figure Lengend Snippet: Representative image of immunostaining of human carotid atherosclerotic plaque. Expression of CARD8 staining (brown/DAB) was evident in endothelial cells is shown using the arrows ( A ), moderately in smooth muscle cells (red/SMA) (as shown with the arrow ( B ) and CD68 (red) positive macrophages (as shown with the arrow ( C ). Expression of CARD8 staining (brown/DAB) in the SMA ( D ) and CD68 ( E ) positive cells in the intimal region of lesions. Left Panel: Magnification, × 2 and Scale bar: 500 μm; Right Panel: Magnification, × 30 (D&E × 20) and Scale bar: 50 μm. Full images are available as Supplementary material.

Article Snippet: The staining was performed using primary antibodies against CARD8 (PAB0281; Abnova Corp, Taipei City, Taiwan; 1:1000/Nordic BioSite AB, Täby, Sweden), CD68 (NCL‐L‐CD68, Novacastra, Newcastle, UK; 1:50), VWF (M0616; Dako, Glostrup, Denmark; 1:50) and smooth muscle actin (SMA; M0851; Dako; 1:500) diluted in Da Vinci Green (Biocare Medical) and incubated for 1 h at room temperature.

Techniques: Immunostaining, Expressing, Staining

Journal: Scientific Reports

Article Title: Expression of CARD8 in human atherosclerosis and its regulation of inflammatory proteins in human endothelial cells

doi: 10.1038/s41598-020-73600-4

Figure Lengend Snippet: Correlation analysis using microarray data from BiKE study. CARD8 expression positively correlated with the expression of CD163 ( A ), and VWF ( B ), in human carotid atherosclerotic plaques. Solid line represent the trend line and the dashed lines represent the 95% confidence intervals for the trend lines.

Article Snippet: The staining was performed using primary antibodies against CARD8 (PAB0281; Abnova Corp, Taipei City, Taiwan; 1:1000/Nordic BioSite AB, Täby, Sweden), CD68 (NCL‐L‐CD68, Novacastra, Newcastle, UK; 1:50), VWF (M0616; Dako, Glostrup, Denmark; 1:50) and smooth muscle actin (SMA; M0851; Dako; 1:500) diluted in Da Vinci Green (Biocare Medical) and incubated for 1 h at room temperature.

Techniques: Microarray, Expressing

Journal: Scientific Reports

Article Title: Expression of CARD8 in human atherosclerosis and its regulation of inflammatory proteins in human endothelial cells

doi: 10.1038/s41598-020-73600-4

Figure Lengend Snippet: Expression of CARD8 mRNA and protein in control and CARD8 knock down HUVECs. The knock down of CARD8 significantly reduced the mRNA ( A ) and protein levels ( B ) of CARD8. Representative protein bands are shown in ( C ). The full image of ( C ) is shown as Supplementary data. Data is presented as mean ± SD for n = 3–6 in each group. p value **** p < 0.0001 is compared to control.

Article Snippet: The staining was performed using primary antibodies against CARD8 (PAB0281; Abnova Corp, Taipei City, Taiwan; 1:1000/Nordic BioSite AB, Täby, Sweden), CD68 (NCL‐L‐CD68, Novacastra, Newcastle, UK; 1:50), VWF (M0616; Dako, Glostrup, Denmark; 1:50) and smooth muscle actin (SMA; M0851; Dako; 1:500) diluted in Da Vinci Green (Biocare Medical) and incubated for 1 h at room temperature.

Techniques: Expressing, Control, Knockdown

Journal: Scientific Reports

Article Title: Expression of CARD8 in human atherosclerosis and its regulation of inflammatory proteins in human endothelial cells

doi: 10.1038/s41598-020-73600-4

Figure Lengend Snippet: Subcellular localization of CARD8 in HUVEC. CARD8 expression in HUVECs treated with control/scramble siRNA ( A ) or CARD8 siRNA ( B ). CARD8 expression is indicated in green , f-actin in red and nucleus are stained blue. Magnification, × 40 and Scale bar: 20 μm. Full images are available as Supplementary material.

Article Snippet: The staining was performed using primary antibodies against CARD8 (PAB0281; Abnova Corp, Taipei City, Taiwan; 1:1000/Nordic BioSite AB, Täby, Sweden), CD68 (NCL‐L‐CD68, Novacastra, Newcastle, UK; 1:50), VWF (M0616; Dako, Glostrup, Denmark; 1:50) and smooth muscle actin (SMA; M0851; Dako; 1:500) diluted in Da Vinci Green (Biocare Medical) and incubated for 1 h at room temperature.

Techniques: Expressing, Control, Staining

Journal: Scientific Reports

Article Title: Expression of CARD8 in human atherosclerosis and its regulation of inflammatory proteins in human endothelial cells

doi: 10.1038/s41598-020-73600-4

Figure Lengend Snippet: Differentially regulated proteins after CARD8 knock down (CARD8 KD) in HUVECs using Olink proteomics panels. Volcano plot displaying differential protein expression in the lysate (CVD II ( A ) and CVDIII ( B ) panels) and in the culture medium [Inflammation panel ( C )]. Colors represent FDR levels (red, FDR ≤ 1%; green, FDR ≤ 5%; blue, FDR ≤ 10%; black, FDR > 10%). The labeled dots represent proteins that were differentially expressed in CARD8 knock down versus control HUVECs (FDR ≤ 10%). ( D ) The protein–protein interaction network as analyzed by String software. Proteins in the STRING software corresponds to the following: TNFRSF10B = TRAIL-R2; F2R = PAR-1; THBD = TM; TNFRSF1A = TNF-R1; TNFSF12 = TWEAK; HSPB1 = HSP27; CCL2 = MCP-1; PLAT = tPA; CXCL8 = IL-8; EIF4EBP1 = 4E-BP1; CCL7 = MCP3; ANGPT1 = ANG1: LDLR = LDL receptor; SIRPA = SHPS1; TNFRSF11B = OPG; PLAU = uPA. The red, violet and green nodes represents proteins involved in inflammatory response, cytokine-mediated signaling pathway and immune system process respectively. The colored lines represent the different possible association between the proteins. A red line indicates the presence of fusion evidence; a green line indicates neighborhood evidence; a blue line indicates co-occurrence evidence; a purple line indicates experimental evidence; a yellow line indicates text-mining evidence; a light blue line indicates database evidence; and a black line indicates co-expression evidence.

Article Snippet: The staining was performed using primary antibodies against CARD8 (PAB0281; Abnova Corp, Taipei City, Taiwan; 1:1000/Nordic BioSite AB, Täby, Sweden), CD68 (NCL‐L‐CD68, Novacastra, Newcastle, UK; 1:50), VWF (M0616; Dako, Glostrup, Denmark; 1:50) and smooth muscle actin (SMA; M0851; Dako; 1:500) diluted in Da Vinci Green (Biocare Medical) and incubated for 1 h at room temperature.

Techniques: Knockdown, Expressing, Labeling, Control, Software

Journal: Scientific Reports

Article Title: Expression of CARD8 in human atherosclerosis and its regulation of inflammatory proteins in human endothelial cells

doi: 10.1038/s41598-020-73600-4

Figure Lengend Snippet: Gene expression levels of selected genes regulated by CARD8. The knock down of CARD8 significantly reduced the expression of CXCL1 ( p = 0.0034), IL6 ( p < 0.0001), CXCL6 ( p < 0.0001), and MCP-1 ( p = 0.0066), when compared to the control. The expression of PDGF-A ( p = 0.0066) was significantly upregulated after the knock down of CARD8 when compared to control. Data are representative of samples from 3 independent experiments and displayed as mean ± SD.

Article Snippet: The staining was performed using primary antibodies against CARD8 (PAB0281; Abnova Corp, Taipei City, Taiwan; 1:1000/Nordic BioSite AB, Täby, Sweden), CD68 (NCL‐L‐CD68, Novacastra, Newcastle, UK; 1:50), VWF (M0616; Dako, Glostrup, Denmark; 1:50) and smooth muscle actin (SMA; M0851; Dako; 1:500) diluted in Da Vinci Green (Biocare Medical) and incubated for 1 h at room temperature.

Techniques: Expressing, Knockdown, Control

Journal: Scientific Reports

Article Title: Expression of CARD8 in human atherosclerosis and its regulation of inflammatory proteins in human endothelial cells

doi: 10.1038/s41598-020-73600-4

Figure Lengend Snippet: Correlation between gene expression of CARD8 and genes associated to inflammatory response and cell migration in human atherosclerotic lesions. CARD8 correlated with ( A ) CXCL1 (r = 0.48, p = 0.000000088); ( B ) CXCL6 (r = 0.31, p = 0.0022); ( C ) MCP-1 (r = 0.28, p = 0.011); ( D ) ALCAM (r = 0.26, p = 0.026); and ( E ) PDGFA (r = −0.38, p = 0.000062); ( F ) IL6 (r = 0.29; p = 0.00082).

Article Snippet: The staining was performed using primary antibodies against CARD8 (PAB0281; Abnova Corp, Taipei City, Taiwan; 1:1000/Nordic BioSite AB, Täby, Sweden), CD68 (NCL‐L‐CD68, Novacastra, Newcastle, UK; 1:50), VWF (M0616; Dako, Glostrup, Denmark; 1:50) and smooth muscle actin (SMA; M0851; Dako; 1:500) diluted in Da Vinci Green (Biocare Medical) and incubated for 1 h at room temperature.

Techniques: Expressing, Migration